Plaque Formation by 55 Rhinovirus Serotypes
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چکیده
منابع مشابه
Plaque formation by 55 rhinovirus serotypes.
Plaque production by 55 rhinoviruses in several lines of HeLa cells is reported. Forty-nine types produced macroscopic plaques under the conditions described previously employing 30 mM MgCl(2) and 30 mug/ml of DEAE-dextran in overlay and M HeLa cells. Basically three kinds of plaques were observed: clear large and intermediate sized plaques and small turbid plaques. Five rhinoviruses produced p...
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A sensitive, reliable plaque assay system is described for five rhinoviruses using freshly prepared methylcellulose overlay and human embryonic diploid cells. Circular plaques with irregular edges, 2 mm in size, were formed by rhinoviruses 1A, 2, 6, and 13 after 6 or 7 days of incubation. A fifth rhinovirus, 17, formed a 1- to 2-mm feather plaque after 14 days of incubation. Plaque counts of rh...
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We have completed production in rabbits of potent antisera to the 90 classified rhinovirus serotypes by using methods previously described (M. K. Cooney and G. E. Kenny, Proc. Soc. Exp. Biol. Med. 133:645-650, 1970). Systematic testing by neutralization tests has revealed significant numbers of cross-relationships among rhinovirus types, some of which have already been reported. Herein, our obs...
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Fiala, Milan (University of Washington, Seattle), and George E. Kenny. Enhancement of rhinovirus plaque formation in human heteroploid cell cultures by magnesium and calcium. J. Bacteriol. 92:1710-1715. 1966.-A reproducible macroplaque assay for six M and three H strains of rhinoviruses has been developed in several human heteroploid cell lines. Plaques were produced only with suitable solidify...
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The cytopathic changes produced by bluetongue virus were first observed by Haig, McKercher & Alexander (1956) in monolayer roller tube cultures of primary lamb kidney ce ll s. Subsequently, Fernandes (1959) described the growth and cytopathic effects of a virulent as well as a homologous egg-adapted strain at the thirtysixth passage level, in cultures of human amnion (Fernandes), Chang liver, c...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1968
ISSN: 0003-6919
DOI: 10.1128/aem.16.10.1445-1450.1968